High-throughput screenings

A.Y. 2020/2021
Overall hours
Learning objectives
Expected learning outcomes
Course syllabus and organization

Single session

Teaching methods.
Lessons, webinars, seminars and Journal Clubs will be held in the classroom if possible, according to Government, Region and University rules. All lessons will be video-recorded and made available through the Ariel platform. If necessary, lessons will be held through Microsoft Teams (or other remote platform) and will be available both synchronously at the scheduled time, and asynchronously through Ariel. Laboratory practices will be held according to the rules of the hosting laboratories, if suitably equipped for the COVID emergency, otherwise they will be substituted with live or recorded video tutorials.

Program and reference material.
The program of the lectures will not be changed. All supporting material will be made available through the Ariel platform.

Methods of learning verification and evaluation criteria.
Exams will be held in preferentially in the classrooms, as explained in "Examination Modalities". Alternatively, the examination will take place using the Microsoft Teams or other remote platform.
Course syllabus
· The basis of High throughput screening:
Target identification and Assay development
Automation and miniaturization, liquid handling,
Detection and data analysis
· Computational high throughput screening
· Biochemical High throughput screening
· Phenotypic screenings and cell-based high throughput screening
High-content versus high-throughput
· In vivo based high throughput screening
· Small molecule-based screenings and drug discovery in academy vs an industrial environment
· Genetic high throughput screenings
RNAi/shRNA-based screenings
CRISPR-Cas9 based screenings
· New approaches:
iPSC and whole organ modeling
Prerequisites for admission
No prior knowledge is required
Teaching methods
Lectures will be accompanied by practicals, group work and time for reflection and collective discussion.
Teaching Resources
We will not use a main textbook, but provide the students with:
· All of the slides presented during the course
· PDF version of all of the articles/reviews presented during the course
· Web addresses of all of the relevant sites (presented during the course)
Assessment methods and Criteria
-Written test (50% of the evaluation), with 2 open questions regarding the topics presented in the course.
-During the course, the students will be divided in small teams (2-3) to prepare journal clubs which will be discussed with the teacher (50% of the evaluation).
Results will be communicated to the students individually by e-mail. The test can be discussed with the Course Supervisor, by appointment.
Practicals: 16 hours
Lessons: 40 hours
Professors: Minucci Saverio, Santaguida Stefano, Varasi Mario