Experimental medicine

Doctoral programme (PhD)
A.Y. 2019/2020
Study area
Medicine and Healthcare
Doctoral programme (PhD)
Dipartimento di Biotecnologie mediche e medicina traslazionale - Via L. Vanvitelli, 32 - 20129 Milano
PhD Coordinator
Experimental medicine develops and applies technology and biological models for studying pathogenic mechanisms in human disease. It involves various disciplines (biochemistry, biology, molecular biology, anatomy, physiology, pathology, immunology, genetics and microbiology/virology) that are providing a rapidly growing understanding of the mechanisms that enable the development of pathological processes. The course aims to acquaint students with the technologies and research methodologies in the area of biomedicine and biotechnology based on in vitro and in vivo experimental models and '-omic' approaches, and on their application to the study of cellular and molecular mechanisms involved in the pathogenesis of human disease. The final objective is the formation of high-level scientists able to combine an understanding of biological processes and pathogenic mechanisms through the application of advanced experimental techniques. The programme's specific objectives are:
1) develop the ability to define problems and devise experiments to solve them in accordance with scientific standards;
2) study biochemical, molecular and cellular mechanisms in physiopathology using advanced technologies;
3) develop the ability to critically assess scientific literature;
4) provide solid, up-to-date knowledge in the specific field;
5) teach how to conduct independent research, and inform on related ethical implications.
Tutte le classi di laurea magistrale
Dipartimento di Biotecnologie mediche e medicina traslazionale - Via L. Vanvitelli, 32 - 20129 Milano
Title Professor(s)
Role of HIF-1alpha in muscle regeneration
L. Anastasia
Unravel the molecular links between aging and human diseases
Requisiti: Skills in molecular biology, biochemistry, cell culture. Basic skills in bioinformatics and biostatistics. Availability  of training abroad.
Epigenetic mechanisms implicated in environmental stress susceptibility and resiliency
New regenerative approaches for spinal cord injury regeneration
Requisiti: Any experience in molecular biology, biochemistry, cell culture, cell metabolism is an advantage. Motivated students with a strong commitment to basic and translational research are invited to apply. The candidate should have well-developed social skills, a hands-on attitude and be able to work in a team. The candidate should have good communication skills in spoken and written English
Generation of zebrafish models for the pathogenesis understanding of endocrine diseases
Identification and characterization of Atypical Chemokine Receptor 2 (ACKR2) expression and function in extracellular vesicles derived from human placenta in both physiological and pathological pregnancies
Role of macrophage iron release in carcinogenesis
Requisiti: Good knowledge of the major techniques and experimental approaches of cell and molecular biology
In search of the molecular basis of sarcopenia: from mice to myogenic cells in culture
Requisiti: Cell culture, Western Blotting, Nucleic acids extraction, RealTime-PCR, gene silencing and gene editing.
Obesity and cardiovascular disease: the role of the receptor for advanced glycation end products (RAGE) in heart steatosis
Requisiti: Previous expertise in cellular and molecular biology: cell cultures, real time RT-PCR, Western Blot, IHC, flow cytometry, immunoenzymatic assays
Natural killer-dendritic cell crosstalk in human glioma microenvironment
Omic approaches and in vitro cell models to dissect the dysregulated pathways of  Mendelian chromatin intellectual disability syndromes to appoint biomarkers of neurolodevelopmental dysfunction
Use of induced pluripotent stem cells iPS for the study of Autonomic Nervous System (ANS) disease: focus on Congenital Central Hypoventilation Syndrome.
Requisiti: The candidate should have a strong background in tissue culture. The candidate should have a background in molecular biology, with emphasis on nucleic acid manipulation (plasmid costruction), mRNA quantitation (RT-PCR), Western blotting, and immunofluorescence.
The role of Protocadherin-19 in neurodevelopment unraveled through the analysis of synapse architecture in neurons from Pcdh9 fl/fl mice
Study of the biological effect of UHMW supplemented with Vitamin E: in vitro evaluation of the bone response
Requisiti: Basic experties in molecular biology, cell coltures, ELISA, saggi biochimici di stress ossidativo
Neural stem precursor cell therapy: a novel therapeutic approach for the treatment of Rett syndrome
Novel macrophage tetraspan molecules: biological relevance immune responses and therapeutic exploitation
The interplay between cellular aging and immune response in human colorectal liver metastasis: senescence as a new prognostic marker?
Understanding the complex relationship between genetic, environment, epigenetics and microbiota composition in the development and prognosis of autoimmune diseases.
At the borders between immune tolerance and autoimmunity: disclosing the mechanisms boosting the expansion of auto-reactive hepatic Natural Killer cells in Primary Biliary Cholangitis
Molecular mechanisms underlying the association between psychiatric disorders and inflammation as potential pharmacological targets
Requisiti: Expertise in gene and protein expression analyses.
Dissecting the pathogenetic role of deregulated non-coding transcripts associated with DIS3 gene mutations in Multiple Myeloma
Requisiti: Experience of basic molecular and cellular biology methods. Experience of transcriptomic analyses.
Multicellular spheroids: a new model for thyroid cancer studies and drug screening.
Study of molecular mechanisms mediating the antiproliferative effects of dopamine receptor type 2 (DRD2) in different types of pituitary tumors: role of Akt pathway and β-arrestins involvement.
Requisiti: Cell culture; cell transfection and silencing; protein detection (western blot, co-/immunoprecipitation); cell imaging, biochemical assays (cell proliferation, apoptosis, migration, invasion, hormone secretion); nucleic acid extraction, PCR.
Investigation of pathomechanisms associated to amyotrophic lateral sclerosis in patient-derived cells
Requisiti: Expertise in molecular biology and cell biology
Role of osteoclast-iron in the pathogenesis of osteoporosis
The role of sphingolipids in the molecular communication between microglia and neurons in neurodegeneration
Requisiti: Strong interest in basic and translational biomedical research; basic skills in biochemistry and molecular biology; experience in cell cultures; good knowledge of English language (written and oral).
Unravelling the routes of neurodegeneration and demyelination involved in Krabbe leukodystrophy
Requisiti: Cell cultures procedures; basic biochemical and molecular biology methods.

Courses list

Courses or activities Professor(s) ECTS Total hours Language
Bionformatics tools web-based to study omic data 2 12 English
Courses or activities Professor(s) ECTS Total hours Language
Animal models of human disease: Zebrafish as an animal model to study human diseases 2 12.5 English
Courses or activities Professor(s) ECTS Total hours Language
New cellular and molecular biomarkers in cardiovascular disease 3 16 English
Translational potential of cell metabolism as new pharmacological target 3 15 English
Courses or activities Professor(s) ECTS Total hours Language
Flow cytometry in biomedical research 4 55 English
Courses or activities Professor(s) ECTS Total hours Language
Biomedical Statistics 2
4 20 English